流式细胞术
What is 流式细胞术?
Flow cytometry uses fluorescent probes to identify and characterize 细胞 or particles in suspension (e.g. 细胞, nuclei or chromosomes) by virtue of size, granularity and fluorescence (auto-fluorescence or fluorescence after immunolabelling or staining). Cells or particles tagged with fluorescent molecules enter the flow cytometer via a fluid stream. 的 细胞 then pass by a laser, which emits a specific wavelength of light. 的 fluorescent probes are excited by the laser and then emit light. 的 fluorescent signal is detected and amplified, then translated into an electronic signal, which is sent to the computer. 的 result is a visual presentation describing an individual or group of cellular events. 的 细胞 or particles can be separated by sorting, or the information can be collected and analyzed.
![Bio-Rad S3](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/flow-cytometry/_jcr_content/par/responsive_columns/col1/bc_image_content/image.img.jpg/1642728675545.jpg)
的 Bio-Rad S3 is located in Higgins 454. Self-use and available 24/7 after training.
![BD FACSAria Cell Sorter](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/flow-cytometry/_jcr_content/par/responsive_columns/col1/bc_image_content_935939022/image.img.jpg/1642728669192.jpg)
的 BD FACSAria Cell Sorter is located is Higgins 458. Operator only and available Monday-Friday, 10-4.
![BD FACSCanto™ Flow Cytometer](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/flow-cytometry/_jcr_content/par/responsive_columns/col2/bc_image_content/image.img.jpg/1642728694349.jpg)
的 BD FACSCanto™ Flow Cytometer is located in Higgins 454. Self-use and available 24/7 after training.
Advances in cell sorting instrumentation have enabled this technology to be applied to a wider array of cell biology areas, 比如血液学, 免疫学, 微生物学, 细胞信号, 和寄生虫学.
Facility Documentation
Please note that all work performed in BC core facilities and recharge centers should always be appropriately acknowledged. If you are publishing or presenting data acquired in BC core facilities and recharge centers, please include the following statement in the Acknowledgement section of your manuscript/poster/presentation, "的 authors would like to thank the 电子游戏软件
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![帕特里克Autissier](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/flow-cytometry/_jcr_content/bottompar/bc_padded_section/par/bc_padded_section/par/responsive_columns/col1/bc_image_content/image.img.jpg/1644772936963.jpg)
帕特里克·奥蒂斯耶博士.D.
Manager, 流式细胞术 Facility
I am a biologist with over 25 years of flow cytometry and cell sorting experience. 我获得了博士学位.D. 从C开始.N.A.M. 在巴黎, 法国, where I developed a 12-color flow cytometry panels to study immune 细胞 in both humans and monkeys. I authored or co-authored more than 40 scientific articles.
My family and I moved to the US in 2001, where I was hired by the Division of Viral Pathogenesis at Beth Israel Deaconess Medical Center in Boston, to run the Cell Sorting Facility. I worked there for 7 years prior to joining 电子游戏软件 where I am in charge of the 流式细胞术 Core facility.
My most important contribution as a technical expert and collaborator has led to the discovery of the TRIM5α protein (capable of blocking HIV infection in monkey 细胞), a groundbreaking advance in AIDS research published by M. Stremlau in the journal Nature in 2004. This study has been widely reported in the mainstream press and has been cited over 800 times in scientific journals since publication. Over the last 18 years, I have acquired a broad knowledge in Immunology, first at the Division of Viral Pathogenesis at Beth Israel Deaconess Medical Center, 在电子游戏软件. 第一个, I worked extensively on measuring immune response on lymphocyte subsets during HIV or SIV infection studies in humans and monkeys. 的n at 电子游戏软件, I focused my work on monocyte/macrophage subset, a cell population thought to bring HIV or SIV in the brain that induce encephalitis and neuronal damage.
As a Director of the BC flow cytometry facility, I interact with many researchers and students. In addition, I manage the scheduling of the facility, the budget and the ordering of reagents. 最后, as a collaborator I helped many researchers designing their flow cytometry experiment so that their research project would be successful.
流式细胞术
帕特里克·奥蒂斯耶博士.D
导演,流
Cytometry Core Facility
617-552-1417
autissie@ycdwkj666.com
Higgins Hall: Room 445A